Browsing by Author "Garlapati, Vijay Kumar"

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    Comprehensive review on biotechnological production of hyaluronic acid: status, innovation, market and applications
    (Taylor and Francis, 2022-04-18) Ucm, Ruschoni; Aema, Mera; Lhb, Zamudio; Kumar, Vinod; Taherzadeh, Mohammad J.; Garlapati, Vijay Kumar
    The growing, existing demand for low-cost and high-quality hyaluronic acid (HA) needs an outlook of different possible production strategies from renewable resources with the reduced possibility of cross-infections. Recently, the possibility of producing HA from harmless microorganisms appeared, which offers the opportunity to make HA more economical, without raw material limitations, and environmentally friendly. HA production is mainly reported with Lancefield Streptococci A and C, particularly from S. equi and S. zooepidemicus. Various modes of fermentation such as batch, repeated batch, fed-batch, and continuous culture have been investigated to optimize HA production, particularly from S. zooepidemicus, obtaining a HA yield of 2.5 g L−1 – 7.0 g L−1. Among the different utilized DSP approaches of HA production, recovery with cold ethanol (4°C) and cetylpyridinium chloride is the ideal strategy for lab-scale HA production. On the industrial scale, besides using isopropanol, filtration (0.22 um), ultrafiltration (100 kDa), and activated carbon absorption are employed to obtain HA of low molecular weight and additional ultrafiltration to purify HA of higher MW. Even though mature technologies have already been developed for the industrial production of HA, the projections of increased sales volume and the expansion of application possibilities require new processes to obtain HA with higher productivity, purity, and specific molecular weights. In this review, we have put forth the progress of HA technological research by discussing the microbial biosynthetic aspects, fermentation and downstream strategies, industrial-scale scenarios of HA, and the prospects of HA production to meet the current and ongoing market demands.
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    Paradigm of integrative OMICS of microbial technology towards biorefinery prospects
    (Elsevier, 2024-05-11) Jacob, Samuel; Rajeswari, Gunasekaran; Rai, Awantika; Tripathy, Sushree Shweta; Gopal, Swathy; Das, Eeshita; Kumar, Vinod; Jeevan Kumar, S. P.; Aminabhavi, Tejraj M.; Garlapati, Vijay Kumar
    Climate change, finite natural resources, and increasing population necessitate producing sustainable energy with positive economic growth. Recent advances in OMICS coupled with genome editing and synthetic biology have paved the way for the development of sustainable technologies. These techniques help identify critical genes/pathways and re-construct and redesign biological pathways to develop eco-friendly and economically viable industrial metabolites. With the help of microbial technology, biorefinery-related research is actively pursued in many countries to develop microbial strains, producing varied value-added biochemicals and biofuels. The application of multi-omics data in deciphering key genes, their manipulation, and outcomes implies the domain's potential to find new horizons in biorefineries using microbial factories to produce various biofuels and biorefinery products. This review illustrates OMICS role in developing industrial chemicals and microbial biorefineries. Besides, prospects for genome editing and synthetic biology have been elucidated.