Browsing by Author "Thomas, Dominic James"

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    Bioproduction of xylitol by oleaginous yeast yarrowia lipolytica.
    (Cranfield University, 2020-01) Thomas, Dominic James; Medina-Vayá, Ángel; Veerheecke-Vaessen, Carol
    Xylitol is a commercially important chemical with multiple applications in the food and pharmaceutical industries. According to the US Department of Energy, xylitol is one of the top twelve platform chemicals that can be produced from biomass. The chemical method for xylitol synthesis is, however, expensive and energy- intensive. In contrast, the biological route using microbial cell factories offers a potentially cost-effective alternative process. The bioprocess occurs under ambient conditions and makes use of biocatalysts and biomass which can be sourced from renewable carbon originating from a variety of cheap waste feedstocks. In this study, the biotransformation of xylose to xylitol was investigated using Yarrowia lipolytica, an oleaginous yeast which, in this study was firstly grown on a glycerol/glucose medium for the screening of a co- substrate, followed by a media optimisation in shake flasks, scale-up studies in a bioreactor and then downstream studies where done on the processing of xylitol. A two-step medium optimization was employed using a central composite design and an artificial neural network coupled with a genetic algorithm. The yeast amassed a concentration of 53 g/L of xylitol whilst using pure glycerol (PG) and xylose media, with a bioconversion yield of 0.97 g/g. Similar results were obtained when PG was substituted with crude glycerol (CG) from the biodiesel industry (titre: 51 g/L; yield: 0.92 g/g). Even when xylose from sugarcane bagasse hydrolysate was used as opposed to pure xylose, a xylitol yield of 0.54 g/g was achieved. The xylitol was successfully crystallized from the PG/xylose and CG/xylose fermentation broths with a recovery yield of 40 and 35 %, respectively. To the best of the author’s knowledge, this study demonstrates for the first time, the potential of using Y. lipolytica as a microbial cell factory for xylitol synthesis from inexpensive feedstocks. The results obtained are competitive with other xylitol producing organisms.