Browsing by Author "Vivek, Narisetty"

Now showing 1 - 6 of 6
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    Isobutanol production by Candida glabrata – A potential organism for future fuel demands
    (Elsevier, 2021-08-13) Lakshmi, Nair M.; Binoop, Mohan; Salini, Chandrasekharan Nair; Vivek, Narisetty; Sindhu, Raveendran; Pandey, Ashok; Binod, Parameswaran
    Due to global concern on the sustainability of energy from fossil fuels, isobutanol as a biofuel has urged attention in recent years due to its high octane number, higher blending capacity, low vapour pressure and higher energy content. The present study reports a novel wild strain, Candida glabrata, which produces isobutanol under submerged fermentation condition. Different process parameters have been optimized by both conventional as well as statistical methods. The production of isobutanol is enhanced in the presence of valine and this attribute the degradation pathway of valine is highly depend on isobutanol production. Under optimized condition, the yield of isobutanol is increased from 0.19 g/L to 0.96 g/L. This strain has not yet reported for the production of isobutanol.
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    Lignocellulose in future biorefineries: strategies for cost-effective production of biomaterials and bioenergy
    (Elsevier, 2021-10-28) Reshmy, R.; Philip, Eapen; Madhavan, Aravind; Sirohi, Ranjna; Pugazhendhi, Arivalagan; Binod, Parameswaran; Awasthi, Mukesh Kumar; Vivek, Narisetty; Kumar, Vinod; Sindhu, Raveendran
    Lignocellulosic biomass has been emerging as a biorefinery precursor for variety of biofuels, platform chemicals and biomaterials because of its specific surface morphology, exceptional physical, chemical and biological characteristics. The selection of proper raw materials, integration of nano biotechnological aspects, and designing of viable processes are important to attain a cost-effective route for the development of valuable end products. Lignocellulose-based materials can prove to be outstanding in terms of techno-economic viability, as well as being environmentally friendly and reducing effluent load. This review should facilitate the identification of better lignocellulosic sources, advanced pretreatments, and production of value-added products in order to boost the future industries in a cleaner and safer way.
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    Molecular biology interventions for activity improvement and production of industrial enzymes
    (Elsevier, 2020-12-24) Bhatia, Shashi Kant; Vivek, Narisetty; Kumar, Vinod; Chandel, Neha; Thakur, Meenu; Kumar, Dinesh; Yang, Yung-Hun; Pugazhendhi, Arivalagan; Kumar, Gopalakrishnan
    Metagenomics and directed evolution technology have brought a revolution in search of novel enzymes from extreme environment and improvement of existing enzymes and tuning them towards certain desired properties. Using advanced tools of molecular biology i.e. next generation sequencing, site directed mutagenesis, fusion protein, surface display, etc. now researchers can engineer enzymes for improved activity, stability, and substrate specificity to meet the industrial demand. Although many enzymatic processes have been developed up to industrial scale, still there is a need to overcome limitations of maintaining activity during the catalytic process. In this article recent developments in enzymes industrial applications and advancements in metabolic engineering approaches to improve enzymes efficacy and production are reviewed
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    Prospects on bio-based 2,3-butanediol and acetoin production: recent progress and advances
    (Elsevier, 2021-06-29) Maina, Sofia; Prabhu, Ashish A.; Vivek, Narisetty; Vlysidis, Anestis; Koutinas, Apostolis; Kumar, Vinod
    The bio-based platform chemicals 2,3-butanediol (BDO) and acetoin have various applications in chemical, cosmetics, food, agriculture, and pharmaceutical industries, whereas the derivatives of BDO could be used as fuel additives, polymer and synthetic rubber production. This review summarizes the novel technological developments in adapting genetic and metabolic engineering strategies for selection and construction of chassis strains for BDO and acetoin production. The valorization of renewable feedstocks and bioprocess development for the upstream and downstream stages of bio-based BDO and acetoin production are discussed. The techno-economic aspects evaluating the viability and industrial potential of bio-based BDO production are presented. The commercialization of bio-based BDO and acetoin production requires the utilization of crude renewable resources, the chassis strains with high fermentation production efficiencies and development of sustainable purification or conversion technologies.
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    Recent advances in microbial biosynthesis of C3 – C5 diols: Genetics and process engineering approaches
    (Elsevier, 2020-12-13) Vivek, Narisetty; Hazeen, Sulfath Hakkim; Alphy, Maria Paul; Kumar, Vinod; Magdouli, Sara; Sindhu, Raveendran; Pandey, Ashok; Binod, Parameswaran
    Diols derived from renewable feedstocks have significant commercial interest in polymer, pharmaceutical, cosmetics, flavors and fragrances, food and feed industries. In C3-C5 diols biological processes of 1,3-propanediol, 1,2-propanediol and 2,3-butanediol have been commercialized as other isomers are non-natural metabolites and lack natural biosynthetic pathways. However, the developments in the field of systems and synthetic biology paved a new path to learn, build, construct, and test for efficient chassis strains. The current review addresses the recent advancements in metabolic engineering, construction of novel pathways, process developments aimed at enhancing in production of C3-C5 diols. The requisites on developing an efficient and sustainable commercial bioprocess for C3-C5 diols were also discussed
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    Sweet sorghum juice as an alternative carbon source and adaptive evolution of Lactobacillus brevis NIE9.3.3 in sweet sorghum juice and biodiesel derived crude glycerol to improve 1, 3 propanediol production
    (Elsevier, 2021-07-21) Alphy, Maria Paul; Anjali, Kodakkattil Babu; Vivek, Narisetty; Thirumalesh, Banjagere Veerabhadrappa; Sindhu, Raveendran; Pugazhendi, Arivalagan; Pandey, Ashok; Binod, Parameswaran
    Sweet sorghum juice (SSJ) is considered as an ideal complement for carbon supplement in ethanol fermentation for its ease of cultivation. Extraction of fermentable sugars from the sweet sorghum is very simple in comparison to lignocellulosic biomass. Hence sweet sorghum is a suitable candidate as a feedstock. In the present study, batch fermentations were carried out using Lactobacillus brevis NIE9.3.3, a facultative anaerobe, isolated through onsite enrichment technique to produce 1,3-propanediol and other co-metabolites, in glucose-glycerol co-fermentation. To make the process more sustainable, the glucose supplemented in the production media was replaced with SSJ. The supplementation of 40 g/L sorghum juice and 40 g/L crude glycerol in the production media resulted in the titre of 25.9 g/L 1, 3-PDO with a volumetric yield of 0.64 g 1,3-PDO/g glycerol. Adaptation of the microorganisms and cultivation under controlled conditions of temperature and substrate concentrations followed by selection was carried out, that is, adaptive evolution. Among the adaptively evolved strains, PD 20.100 has displayed better performance and increased the titres up to 38.4 g/L with a volumetric yield of 0.64 g 1,3-PDO/g glycerol. The industrial applicability of the fermentation process was checked in pilot scale and the production yield was comparable with that of flask scale. The utilization of agricultural and biodiesel industrial waste for the production of 1,3-PDO by a non-pathogenic organism and the strain improvement through ALE for better utilization and conversion of substrates indicates the novelty of this work.