Wang, EnzhenSun, HuiXing, FanZheng, YonghuiChen, PenghuiLyu, TaoLiu, RuotongLi, XinDong, RenjieGuo, Jianbin2025-01-072025-01-072024-12-01Wang E, Sun H, Xing F, et al., (2024) Metagenomic analysis reveals metabolic mechanism of enhancing lignocellulosic anaerobic digestion mediated by CO2/O2-nanobubble water. Bioresource Technology, Volume 414, December 2024, Article number 1316220960-8524https://doi.org/10.1016/j.biortech.2024.131622https://dspace.lib.cranfield.ac.uk/handle/1826/23334Nanobubble water (NW) has been reported to enhance anaerobic digestion (AD), but its influence on the metabolic pathways of microorganisms remains unclear. In this study, the specific methane yields of rice straw in the CO2NW and O2NW treatments increased by 6.9% and 18.3%, respectively. The electron transport system (ETS) and coenzyme F420 activities were enhanced by the addition of NW. Metagenomic analysis showed that the abundances of most enzymes in the acidification were significantly increased by both CO2NW and O2NW. Regarding methanogenesis, CO2NW promoted the expression of genes encoding enzymes of hydrogenotrophic methanogenesis, while O2NW stimulated both the acetoclastic and hydrogenotrophic methanogenesis. With the addition of O2NW, the expressions of modules related to the tricarboxylic acid (TCA) cycle and oxidative phosphorylation were enhanced, resulting in increased ATP production. This study provided fundamental evidence of the metabolic pathways of microorganisms mediated by NW at each stage of AD.Print-ElectronicenAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Biogas productionNanobubblesRice strawMicro-oxygenMicrobial community31 Biological Sciences3106 Industrial BiotechnologyBiogas productionMicro-oxygenMicrobial communityNanobubblesRice strawBiotechnology3001 Agricultural biotechnology3106 Industrial biotechnology3107 MicrobiologyAnaerobiosisLigninCarbon DioxideMetagenomicsMethaneWaterOxygenOryzaCarbon DioxideOxygenWaterMethaneLigninAnaerobiosisMetagenomicsOryzaAnaerobiosisLigninCarbon DioxideMetagenomicsMethaneWaterOxygenOryzaArticle1873-2976554859131622414