Narisetty, VivekZhang, LeZhang, JingxinLin, Carol Sze KiTong, Yen WahShow, Pau LokeBhatia, Shashi KantMisra, AshishKumar, Vinod2022-06-132022-06-132022-06-01Narisetty V, Zhang L, Zhang J, et al., (2022) Fermentative production of 2,3-Butanediol using bread waste – A green approach for sustainable management of food waste. Bioresource Technology, Volume 358, August 2022, Article number 1273810960-8524https://doi.org/10.1016/j.biortech.2022.127381http://dspace.lib.cranfield.ac.uk/handle/1826/18011Bread is Europe’s most wasted food, and the second most wasted food after potatoes in UK. Bread waste (BW) is a clean source of high-quality fermentable sugars. In this study, the potential of Enterobacter ludwigii to accumulate 2,3-butanediol (BDO) from BW was evaluated. Initially, the optimal inoculum size and yeast extract concentration were determined, followed by extraction of sugars from BW using acid and enzymatic hydrolysis. A glucose yield of 330–530 g/kg BW was obtained, and the sugars released were utilised for BDO production by E. ludwigii. The fed-batch cultivation using pure glucose and glucose rich hydrolysates from acid and enzymatic hydrolysis resulted in BDO titres of 144.5, 135.4, and 138.8 g/L, after 96 h, with yield of 0.47, 0.42 and 0.48 g/g yield, respectively. The innovation of the work is valorisation of BW to BDO with a circular biorefining approach and thus, reducing BW disposal and associated environmental burden.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Bread wasteEnterobacter ludwigiiDextrozyme PeakHydrolysate2,3-ButanediolArticle