Zhang, JiongWan, YanjianLi, YuanyuanZhang, QiongfangXu, ShunqingZhu, HuijunShu, Baihua2012-02-032012-02-032011-12-31Jiong Zhang, Yanjian Wan, Yuanyuan Li, Qiongfang Zhang, Shunqing Xu, Huijun Zhu and Baihua Shu. A rapid and high-throughput quantum dots bioassay for monitoring of perfluorooctane sulfonate in environmental water samples. Environmental Pollution, Volume 159, Issue 5, May 2011, Pages 1348-13530269-7491http://dx.doi.org/10.1016/j.envpol.2011.01.011http://dspace.lib.cranfield.ac.uk/handle/1826/6959Currently HPLC/MS is the state of the art tool for environmental/drinking water perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal proliferator-activated receptor-alpha (PPARα), which forms heterodimers with retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay free PFOS in water samples competes with immobilized PFOS in ELISA plates for a given amount of PPARα-RXRα. It can be determined indirectly by immobilizing PPARα-RXRα-PFOS complex to another plate coated with PPARα antibody and subsequent measuring the level of PPARα-RXRα by using biotin-modified PPARα- RXRα probes-quantum dots-streptavidin detection system. The rapid and high- throughput bioassay demonstrated a detection limit of 2.5ngL-1 with linear range between 2.5ngL-1 and 75ngL-1. Detection results of environmental water samples were highly consistent between the bioassay anArticle