Cho, Si-KyungTrchounian, KarenReungsang, AlissaraKumar, VinodIgliński, BartłomiejPiechota, GrzegorzKumar, Gopalakrishnan2025-07-042025-07-042025-08-01Cho S-K, Trchounian K, Reungsang A, et al., (2025) Critical factors influencing biohythane production from research to commercialization. Energy, Volume 328, August 2025, Article number 1362840360-5442https://doi.org/10.1016/j.energy.2025.136284https://dspace.lib.cranfield.ac.uk/handle/1826/24164The shift from fossil-derived energy to clean, renewable sources has accelerated due to the demand for sustainable and commercially viable energy solutions. Another possible clean fuel is biohythane, also known as HCNG, which is a blend of bio–CH4 and bio–H2 with a concentration of 10 and 30 % v/v, respectively. The chemical industries have eventually been utilizing bio–CH4 and bio–H2 extensively because of their high calorific values of 143 and 55 kJ g−1, respectively. The bio–H2 and bio–CH4 have been emerged as promising green energy carriers, offering a broad range of applications in chemical industries, owing to their high calorific value, renewability, and CO2 neutrality. In the pursuit of zero-emission technologies to mitigate the global warming, biohythane is gaining attraction as a potential future fuel. This study explores biohythane production through a sequential two–stage process that converts organic wastes into bio–H2 and subsequently into bio–CH4, offering an ideal pathway for sustainable biohythane generation. Further, this study provides an overview of key developments and applications in two–stage microbial synthesis of bio–CH4 and bio–H2, alongside insights into the demand, supply, and current global status of biohythane production. This study delves into the acidogenic phase, where bio–H2 is produced, and the methanogenic phase, which yields bio–CH4. While detailing the biochemistry, critical factors, challenges, and limitations of an integrated bio–hythane production system are discussed. Finally, the strategies for enhancing bio–H2 and biohythane production and an outlook on their commercialization potential are discussed.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Bio-H-2Bio-CH4Biohythane two-stage pathBES systemsCritical factors4012 Fluid Mechanics and Thermal Engineering40 Engineering4008 Electrical Engineering4017 Mechanical Engineering7 Affordable and Clean Energy13 Climate ActionEnergyArticle1873-6785673100136284328