Salam, FaridahTothill, Ibtisam E.2011-10-112011-10-112009-04-15Faridah Salam, Ibtisam E. Tothill, Detection of Salmonella typhimurium using an electrochemical immunosensor, Biosensors and Bioelectronics, Volume 24, Issue 8, 15 April 2009, Pages 2630-26360956-5663http://dx.doi.org/10.1016/j.bios.2009.01.025http://dspace.lib.cranfield.ac.uk/handle/1826/3396An electrochemical immunosensor based on screen-printed gold working electrode with onboard carbon counter and silver–silver chloride pseudo-reference electrode for Salmonella typhimurium detection is described in this paper. Monoclonal anti-S. typhimurium antibody was immobilized using physical and covalent immobilization via amine coupling of carboxymethyldextran on the surface of the gold working electrode. A direct sandwich enzyme-linked immunosorbent assays (ELISA) format was then developed and optimized using a polyclonal anti-Salmonella antibodies conjugated to horseradish peroxidase (HRP) as the enzyme label. 3,3′,5,5′-Tetramethylbenzidine dihydrochloride (TMB)/H2O2 was used as the enzyme mediator/substrate system. Electrochemical detection was conducted using chronoamperometry at −200 mV vs. onboard screen-printed Ag–AgCl pseudo-reference electrode. The applied potential was selected through the study of the electrochemical behaviour of bare gold electrode with TMB–H2O2–IgG–HRP system. S. typhimurium detection of 5 × 103 cells ml−1 and 20 cells ml−1 was achieved respectively for physical and covalent antibody immobilization. The developed sensor was then compared to a commercial ELISA kit and a chromogenic agar plating method for meat samples analysis. The sensor format shows a promising technology for simple and sensitive detection system for Salmonella contamination. Rapid detection of Salmonella is a key to the prevention and identification of problems relateden-UKImmunosensorScreen-printed gold electrodeSalmonellaElectrochemical detectionFood monitoringArticle