Harrison, Peter J.Chandler, JakeThompson, Andrew J.Bugg, Timothy D. H.2024-08-132024-08-132024-09-17Harrison PJ, Chandler J, Thompson AJ, Bugg TD. (2024) In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays. Methods in Enzymology (Book Series: Mononuclear Non-heme Iron Dependent Enzymes Part B), Volume 704, September 2024, pp. 291-3120076-6879https://doi.org/10.1016/bs.mie.2024.05.012https://dspace.lib.cranfield.ac.uk/handle/1826/22777The article reports methods for the expression and assay of 9-cis-epoxycarotenoid cleavage dioxygenase (NCED), an enzyme involved in the biosynthesis of phytohormone abscisic acid in plants. A method for the preparation of the unstable substrate 9′-cis-neoxanthin from fresh spinach is described. The inhibition of Solanum lycopersicum NCED by a series of aryl hydroxamic acid inhibitors is illustrated, and inhibitors D2 and D4 are assayed against NCED isozymes from Zea mays.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Book chapter1557-7988