Bai, QiangLuo, HongyangShi, ShugaoLiu, ShenWang, LinaDu, FanglinYang, ZhugenZhu, ZhilingSui, Ning2022-01-192022-01-192022-01-10Bai Q, Luo H, Shi S, et al., (2022) AuAg nanocages/graphdiyne for rapid elimination and detection of trace pathogenic bacteria. Journal of Colloid and Interface Science, Volume 613, May 2022, pp. 376-3830021-9797https://doi.org/10.1016/j.jcis.2022.01.046http://dspace.lib.cranfield.ac.uk/handle/1826/17446We prepared a biocompatible AuAg nanocages/graphdiyne @ polyethylene glycol (AuAg/GDY@PEG) composite. The combination of AuAg and GDY to obtain a synergistically enhanced photothermal effect, and the antibacterial effect of GDY and AuAg are used in combined anti-infective therapy. The in vitro antibacterial activity of AuAg/GDY@PEG was investigated, showing an impressive broad-spectrum antibacterial activity with the killing rate > 99.999%. Based on the photothermal conversion ability of AuAg/GDY@PEG, a simple photothermal immunoassay for pathogenic bacteria was successfully established. Sandwich immune response was performed on a microporous plate, the microplate containing the antibody binds specifically to the bacterium being tested, which then binds to the material with the antibody on its surface, and the signal was a change in temperature under 808 nm near-infrared light. The limit of detection (LOD) for S. typhimurium detection is 103 CFU mL−1, with a range of 103–107 CFU mL−1. This method is accurate, rapid and low-cost, which can be used for on-site detection of pathogenic bacteria in food.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Photothermal effectGold-silver nanocagesGraphdiyneImmunoassayAntibacterialArticle