Medina-Vayá, ÁngelValle-Algarra, Francisco M.Jimenez, MisericordiaMagan, Naresh2011-01-182011-01-182010-08-01Angel Medina, Francisco M. Valle-Algarra, Misericordia Jimenez, Naresh Magan; Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media, Journal of Chromatography B, Volume 878, Issue 23, 1 August 2010, Pages 2145-21491570-0232http://dx.doi.org/10.1016/j.jchromb.2010.05.043http://dspace.lib.cranfield.ac.uk/handle/1826/4746A LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in cultures of Fusarium langsethiae in oat-based and other in vitro media. Test media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin stock solutions were added. Different mixtures of extraction solvent (acetonitrile:water and methanol water), extraction times (30', 60' or 90') and drying methods were investigated. Results showed that extraction with methanol: water (80:20, v/v) for 90 min, drying with N-2 and subsequent analysis by LC-DAD was the fastest and most user friendly method for detecting HT-2 and T-2 toxins production by F. langsethiae strains grown on oat-based media at levels of 0.459 and 0.508 mg of toxin/kg of agar, respectively. The proposed method was used to investigate toxin production of 6 F. langsethiae strains from northern Europe and provided clear chromatograms with no interfering peaks in media with and without glycerol as water activity modifier. (C) 2010 Elsevier B.V. All rights reserved.en-UKAnalysis Type A trichothecenes Diode array Cereals performance liquid-chromatography diode-array detection gas-chromatography mass-spectrometry immunoaffinity cleanup fluorescence detection fusarium-langsethiae retention indexes b-trichothecene cerealsArticle