Guerreiro, Antonio R.Chianella, IvaPiletska, Elena V.Whitcombe, Michael J.Piletsky, Sergey A.2012-02-032012-02-032009-04-15Guerreiro AR, Chianella I, Piletska E, Whitcombe MJ, Piletsky SA. (2009) Selection of imprinted nanoparticles by affinity chromatography, Biosensors and Bioelectronics, Volume 24, Issue 8, April 2009, pp. 2740-27430956-5663http://dx.doi.org/10.1016/j.bios.2009.01.013http://dspace.lib.cranfield.ac.uk/handle/1826/6919Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography. Subsequent fractionation of these particles by affinity chromatography allowed the separation of high affinity fractions from the mixture of nanoparticles. Fractions selected this way possess affinity similar to that of natural antibodies (Kd 6.6 × 10−8) M and were also able to discriminate between related functional analogues of the template.en-UKMolecularly imprinted polymerNanoparticleAffinity chromatographyArticle