Browsing by Author "Gopaliya, Deeksha"

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    Integrated fermentative production and downstream processing of L-malic acid by Aspergillus wentii using cassava peel waste
    (Elsevier, 2023-03-28) Gopaliya, Deeksha; Zaidi, Saniya; Srivastava, Nitin; Rani, Bhumika; Kumar, Vinod; Khare, Sunil Kumar
    L-malic acid (L-MA) is an industrially significant chemical with enormous potential. The fungal cell factories could be exploited to harvest it on large scales. In our study, Aspergillus wentii strain (MTCC 1901 T) was explored for L-MA production. Initially, the L-MA production was carried out using glucose with optimization of parameters influencing product accumulation (pH and CaCO3). The fermentation resulted in L-MA titer of 37.9 g/L with 0.39 g/g yield. Then, cassava peel waste (CPW) was used for L-MA production by separate hydrolysis and fermentation. Optimized acidic and enzymatic hydrolysis resulted in glucose release of 0.53 and 0.66 g/g CPW, respectively. The strain accumulated 20.9 g/L and 33.1 g/L L-MA with corresponding yields of 0.25 g/g and 0.34 g/g during batch cultivation using acid and enzyme hydrolysate, respectively. Finally, the produced L-MA was separated using an inexpensive solvent extraction method. Among various solvents used, n-butanol exhibited maximum L-MA extraction efficiency (31%).
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    Microbial itaconic acid production from starchy food waste by newly isolated thermotolerant Aspergillus terreus strain
    (Elsevier, 2021-06-17) Narisetty, Vivek; Prabhu, Ashish A.; Al-Jaradah, Khalid; Gopaliya, Deeksha; Hossain, Abeer H.; Khare, Sunil Kumar; Punt, Peter J.; Kumar, Vinod
    In the present study, we have explored the potential of newly isolated Aspergillus terreus BD strain, which can accumulate itaconic acid (IA) at higher temperature. The shake flask cultivation of thermotolerant strain with medium optimized using Box-Behnken Design at 45 °C resulted in IA accumulation of 28.9 g/L with yield of 0.27 g/g. The enzymatic saccharification of the synthetic food waste (SFW) consisting of potatoes, rice & noodles were optimized using Taguchi method of orthogonal array to maximize the release of fermentable sugar. The maximum glucose release of 0.60 g/g was achieved with 10% biomass loading, 5% enzyme concentration, pH 5.5 and temperature 60 0C. The sugars obtained from SFW was integrated with IA production and maximum IA titer achieved with SFW hydrolysate during bioreactor cultivation was 41.1 g/L with conversion yield of 0.27 g/g while with pure glucose IA titer and yield were 44.7 g/L and 0.30 g/g, respectively.
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    Recent advances in itaconic acid production from microbial cell factories
    (Elsevier, 2021-08-18) Gopaliya, Deeksha; Kumar, Vinod; Khare, Sunil Kumar
    Itaconic acid is an unsaturated organic acid with two carboxyls and one methylene group. The presence of these functional groups, along with a conjugated double bond, makes itaconic acid a versatile molecule with a vast number of applications. Itaconic acid can be produced through chemical as well as biological routes. Aspergillus terreus is the most prevalent microbial cell factory for the biological production of itaconic acid, reaching titers of >100 g/L. However, it suffers from low yield and volumetric productivities leading to high manufacturing costs. The wider applications of itaconic acid can be enabled with a low-cost production process, which can be achieved with cheaper feedstocks and robust cell factories accumulating itaconic acid efficiently. The current review summarizes the recent advances in the biological production of itaconic acid with a focus on the metabolic engineering of prokaryotic and eukaryotic systems for the overproduction of itaconic acid. It comprehensively describes various microbial cell factories with an insight into the pathway leading to itaconic acid production in natural producers like A. terreus and U. maydis. It also discusses the metabolic engineering approaches to improve strain performance in terms of high itaconic acid productivity, less by-product generation, and the ability to utilize unconventional cheap substrates. Moreover, the alternative strategies for the development of non-native producers through genome engineering and the hurdles related to itaconic acid production have been elaborated.
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    Recycling potential of brewer's spent grains for circular biorefineries
    (Elsevier, 2022-12-23) Agrawal, Deepti; Gopaliya, Deeksha; Willoughby, Nicholas; Khare, Sunil K.; Kumar, Vinod
    Brewer's spent grain (BSG) is the major by-product of the brewing industry. BSG is principally composed of carbohydrates and proteins, with substantial amount of lipids. Presently, BSG usage is restricted to low-grade applications such as ruminant feed or landfills. The high volume, nutrient-rich composition, low cost (€35/ton), abundance, and around the year availability, makes it a promising and renewable feedstock for biorefinery development. The current review begins with beer production process, where BSG is produced. Further, it appraises emerging biotechnological advancements and green processes targeting BSG valorisation ensuring maximal resource recovery. Particularly, it illustrates diverse marketable products obtained by repurposing carbohydrate and protein fraction of BSG using either isolated or cascading approach. We believe that this review will encourage more research groups to work on developing innovative technologies for integrated and holistic valorisation of BSG. Inclusive efforts towards reduced water consumption and waste minimisation is further advocated, which are presently primary challenges associated with beer industry. It will leave a significant imprint on environmental sustainability and pave a way for developing circular bio-based economy.