Browsing by Author "Wilson, Sandra"

Now showing 1 - 8 of 8
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    Additive manufacturing and physicomechanical characteristics of PEGDA hydrogels: recent advances and perspective for tissue engineering
    (MDPI, 2023-05-17) Khalili, Mohammad Hakim; Zhang, Rujing; Wilson, Sandra; Goel, Saurav; Impey, Susan A.; Aria, Adrianus Indrat
    In this brief review, we discuss the recent advancements in using poly(ethylene glycol) diacrylate (PEGDA) hydrogels for tissue engineering applications. PEGDA hydrogels are highly attractive in biomedical and biotechnology fields due to their soft and hydrated properties that can replicate living tissues. These hydrogels can be manipulated using light, heat, and cross-linkers to achieve desirable functionalities. Unlike previous reviews that focused solely on material design and fabrication of bioactive hydrogels and their cell viability and interactions with the extracellular matrix (ECM), we compare the traditional bulk photo-crosslinking method with the latest three-dimensional (3D) printing of PEGDA hydrogels. We present detailed evidence combining the physical, chemical, bulk, and localized mechanical characteristics, including their composition, fabrication methods, experimental conditions, and reported mechanical properties of bulk and 3D printed PEGDA hydrogels. Furthermore, we highlight the current state of biomedical applications of 3D PEGDA hydrogels in tissue engineering and organ-on-chip devices over the last 20 years. Finally, we delve into the current obstacles and future possibilities in the field of engineering 3D layer-by-layer (LbL) PEGDA hydrogels for tissue engineering and organ-on-chip devices.
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    Data for: Nanoindentation Response of 3D Printed PEGDA Hydrogels in a Hydrated Environment
    (Cranfield University, 2023-01-27 09:40) Hakim Khalili, Mohammad; J. Williams, Craig; Zhang, Rujing; Wilson, Sandra; Impey, Sue; Aria, Indrat; Goel, Saurav; Dossi, Licia; Afsar, Ashfaq; Duarte martinez, Fabian; Micallef, Christian
    Raw and processed data sets from nanoidentation response of 3D printed hydrogels. 1. Raw data of nanoindentation response. 2. Representative load-displacement curves for each type of hydrogel. 3. Representative data for creep for different types of hydrogels. 4. Representative data for NMR spectras of different types of hydrogels. 5. Representative data for glass transition of different types of hydrogels.
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    Data supporting 'Thermal response of multi-layer UV crosslinked PEGDA hydrogels'
    (Cranfield University, 2023-02-16 11:15) Hakim Khalili, Mohammad; Afsar, Ashfaq; Zhang, Rujing; Wilson, Sandra; Goel, Saurav; Impey, Sue; Aria, Adrianus
    All data sets are raw data from thermoresponse behaviour of hydrogels. 1. Swelling test for multi-150 um hydrogels with 1.8 mg/ml of photoabsorber.2. Swelling test for mono-5 mm hydrogels with 0 mg/ml of photoabsorber.3. Swelling test for multi-20 um hydrogels with 9 mg/ml of photoabsorber.4. Swelling test for mono-3 mm and mono-1.5 mm hydrogels with 0 mg/ml of photoabsorber.5. Cyclic test for multi-150 um hydrogels.6. Dried weight and solid residue weight of all hydrogels samples7. EWC, NWF, NVF-summary for all hydrogel samples8. DSC-TG-Thermogram-All sample types
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    Data supporting: 'Mechanical Behavior of 3D Printed Poly(ethylene glycol) Diacrylate Hydrogels in Hydrated Conditions Investigated Using Atomic Force Microscopy'
    (Cranfield University, 2023-03-21 08:49) Hakim Khalili, Mohammad; Panchal, Vishal; Dulebo, Alexander; Hawi, Sara; Zhang, Rujing; Wilson, Sandra; Dossi, Licia; Goel, S.; Impey, Sue; Aria, Indrat
    1. File AFM-Lines: Raw files for all force-distance curves along with excel file summarizing all the indentions on a single line taken at different height on the surface of the hydrogel. 2. File AFM-Maps: Raw files for all force-distance curves along with excel file summarizing all the indentation maps taken at the middle section on the surface of the hydrogel.
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    Design and development of a polymer patch clamping device
    (Cranfield University, 2010) Wilson, Sandra; Kirby, Paul B.; Saile, Volker
    Patch clamping is considered the gold standard in measuring the bioelectrical activity of a cell. It is used to detect and measure ion transport through ion channels located throughout a cell membrane. Ion movement is crucial to cell viability and cell-to-cell communication. Pharmaceutical companies increasingly target ion channels because of their significance in disease and to help design better targeted drugs. However, the traditional method of patch clamping is cumbersome and is being replaced by planar high throughput screening (HTS) systems. These systems are reaching their limits due to materials and cost of processing; cell handling methods and small varieties of applicable cell types are also issues to be addressed. In this work, the core components of a new kind of planar patch clamping device have been designed and developed, after analysis of currently available HTS systems. This design approaches patch clamping using polymers to overcome some of the limitations in current systems, specifically cell handling and positioning, by using a simple modification technique to provide distinct attractive areas for cell binding. This uniquely allows the culture of both single cells and cell networks to increase the range of cell types that can be measured and circumvents challenges from using suction to pull cells onto measurement holes. The components of the design are a 10 x 10 array of small holes drilled in a polymer then aligned modifications for precise cell placement are added and a planar electrode array for individual addressing of each cell. A study of methods to produce a leak-tight seal required between microfluidic chambers was done. Cell adhesion parameters for the modification techniques were established. The principle viability of this approach was confirmed using the modification technique to culture cells over holes and measure their resistance using a rig developed for this work.
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    Mechanical behavior of 3d printed poly(ethylene glycol) diacrylate hydrogels in hydrated conditions investigated using atomic force microscopy
    (American Chemical Society, 2023-04-05) Hakim Khalili, Mohammad; Panchal, Vishal; Dulebo, Alexander; Hawi, Sara; Zhang, Rujing; Wilson, Sandra; Dossi, Eleftheria; Goel, Saurav; Impey, Susan A.; Aria, Adrianus Indrat
    Three-dimensional (3D) printed hydrogels fabricated using light processing techniques are poised to replace conventional processing methods used in tissue engineering and organ-on-chip devices. An intrinsic potential problem remains related to structural heterogeneity translated in the degree of cross-linking of the printed layers. Poly(ethylene glycol) diacrylate (PEGDA) hydrogels were used to fabricate both 3D printed multilayer and control monolithic samples, which were then analyzed using atomic force microscopy (AFM) to assess their nanomechanical properties. The fabrication of the hydrogel samples involved layer-by-layer (LbL) projection lithography and bulk cross-linking processes. We evaluated the nanomechanical properties of both hydrogel types in a hydrated environment using the elastic modulus (E) as a measure to gain insight into their mechanical properties. We observed that E increases by 4-fold from 2.8 to 11.9 kPa transitioning from bottom to the top of a single printed layer in a multilayer sample. Such variations could not be seen in control monolithic sample. The variation within the printed layers is ascribed to heterogeneities caused by the photo-cross-linking process. This behavior was rationalized by spatial variation of the polymer cross-link density related to variations of light absorption within the layers attributed to spatial decay of light intensity during the photo-cross-linking process. More importantly, we observed a significant 44% increase in E, from 9.1 to 13.1 kPa, as the indentation advanced from the bottom to the top of the multilayer sample. This finding implies that mechanical heterogeneity is present throughout the entire structure, rather than being limited to each layer individually. These findings are critical for design, fabrication, and application engineers intending to use 3D printed multilayer PEGDA hydrogels for in vitro tissue engineering and organ-on-chip devices.
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    Nanoindentation response of 3D printed PEGDA hydrogels in hydrated environment
    (American Chemical Society, 2023-01-20) Hakim Khalili, Mohammad; Williams, Craig J.; Micallef, Christian; Duarte-Martinez, Fabian; Afsar, Ashfaq; Zhang, Rujing; Wilson, Sandra; Dossi, Eleftheria; Impey, Susan A.; Goel, Saurav; Aria, Adrianus Indrat
    Hydrogels are commonly used materials in tissue engineering and organ-on-chip devices. This study investigated the nanomechanical properties of monolithic and multilayered poly(ethylene glycol) diacrylate (PEGDA) hydrogels manufactured using bulk polymerization and layer-by-layer projection lithography processes, respectively. An increase in the number of layers (or reduction in layer thickness) from 1 to 8 and further to 60 results in a reduction in the elastic modulus from 5.53 to 1.69 and further to 0.67 MPa, respectively. It was found that a decrease in the number of layers induces a lower creep index (CIT) in three-dimensional (3D) printed PEGDA hydrogels. This reduction is attributed to mesoscale imperfections that appear as pockets of voids at the interfaces of the multilayered hydrogels attributed to localized regions of unreacted prepolymers, resulting in variations in defect density in the samples examined. An increase in the degree of cross-linking introduced by a higher dosage of ultraviolet (UV) exposure leads to a higher elastic modulus. This implies that the elastic modulus and creep behavior of hydrogels are governed and influenced by the degree of cross-linking and defect density of the layers and interfaces. These findings can guide an optimal manufacturing pathway to obtain the desirable nanomechanical properties in 3D printed PEGDA hydrogels, critical for the performance of living cells and tissues, which can be engineered through control of the fabrication parameters.
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    Thermal response of multi-layer UV crosslinked PEGDA hydrogels
    (Elsevier, 2022-01-07) Khalili, Mohammad Hakim; Afsar, Ashfaq; Zhang, Rujing; Wilson, Sandra; Dossi, Eleftheria; Goel, Saurav; Impey, Susan A.; Aria, Adrianus Indrat
    Poly(ethylene glycol) diacrylate (PEGDA) hydrogels are ubiquitously used in a wide variety of applications in tissue engineering. In this study, the thermal response of multi-layered PEGDA hydrogels was investigated under various conditions of the temperature-controlled environments (8, 20, 37, and 45 °C) through gravimetric and volumetric methods. These multi-layered hydrogels were produced using a computer-controlled projection lithography and compared to the monolithic hydrogels fabricated through bulk photo-crosslinking. It was observed that the volume of multi-layered PEGDA hydrogels increased to about 10% at a temperature of 8 °C, while their volume decreased by 8% and 12% when stored at 37 °C and 45 °C, respectively. PEGDA hydrogel also showed an anisotropic characteristic where the axial dimensional change was about 43% higher than the lateral dimension. This finding is critical to inform the design and fabrication of PEGDA hydrogels to compensate for the axial and lateral volume changes during the application at different temperatures.