Browsing by Author "Zhang, Jing"

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    Droplet microfluidics on analysis of pathogenic microbes for wastewater-based epidemiology
    (Elsevier, 2021-05-18) Ou, Yangteng; Cao, Shixiang; Zhang, Jing; Zhang, Jing; Dong, Weiliang; Yang, Zhugen; Yu, Ziyi
    Infectious diseases caused by pathogenic microbes have posed a major health issue for the public, such as the ongoing COVID-19 global pandemic. In recent years, wastewater-based epidemiology (WBE) is emerging as an effective and unbiased method for monitoring public health. Despite its increasing importance, the advancement of WBE requires more competent and streamlined analytical platforms. Herein we discuss the interactions between WBE and droplet microfluidics and focus on the analysis of pathogens in droplets, which is hard to be tackled by traditional analytical routines. We highlight research works from three aspects, namely, quantitation of pathogen biomarkers in droplets, single-cell analysis in droplets, and living cell biosensors in droplets, with the aim of providing future perspectives on the synergy between WBE and droplet microfluidics
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    Meta-analysis of the effects of 1-methylcyclopropene (1-MCP) treatment on climacteric fruit ripening
    (Nature Publishing Group, 2020-12-03) Zhang, Jing; Ma, Yuanchun; Dong, Chao; Terry, Leon A; Watkins, Christopher B.; Yu, Zhifang; Cheng, Zong-Ming (Max)
    1-Methylcyclopropene (1-MCP) is an inhibitor of ethylene perception that is widely used to maintain the quality of several climacteric fruits during storage. A large body of literature now exists on the effects of 1-MCP on climacteric fruit ripening for different species and environmental conditions, presenting an opportunity to use meta-analysis to systematically dissect these effects. We classified 44 ripening indicators of climacteric fruits into five categories: physiology and biochemistry, quality, enzyme activity, color, and volatiles. Meta-analysis showed that 1-MCP treatment reduced 20 of the 44 indicators by a minimum of 22% and increased 6 indicators by at least 20%. These effects were associated with positive effects on delaying ripening and maintaining quality. Of the seven moderating variables, species, 1-MCP concentration, storage temperature and time had substantial impacts on the responses of fruit to 1-MCP treatment. Fruits from different species varied in their responses to 1-MCP, with the most pronounced responses observed in rosaceous fruits, especially apple, European pear fruits, and tropical fruits. The effect of gaseous 1-MCP was optimal at 1 μl/l, with a treatment time of 12–24 h, when the storage temperature was 0 °C for temperate fruits or 20 °C for tropical fruits, and when the shelf temperature was 20 °C, reflecting the majority of experimental approaches. These findings will help improve the efficacy of 1-MCP application during the storage of climacteric fruits, reduce fruit quality losses and increase commercial value.
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    Reducing arsenic toxicity using the interfacial oxygen nanobubble technology for sediment remediation
    (Elsevier, 2021-09-11) Tang, Ying; Zhang, Meiyi; Zhang, Jing; Lyu, Tao; Cooper, Mick; Pan, Gang
    The arsenic (As)-bearing eutrophic waters may suffer from the dual conditions of harmful algal blooms and release of As, driven by algal-induced hypoxia/anoxia. Here, we investigate the use of interfacial oxygen (O2) nanobubble technology to combat the hypoxia and control As exposure in simulated mesocosm experiments. It was observed that remediation of algal-induced hypoxia at the sediment-water interfaces (SWI) by application of O2 nanobubbles reduced the level of dissolved As from 23.2 μg L−1 to <10 μg L−1 and stimulated the conversion of As(III) to the less toxic As(V) (65–75%) and methylated As (10–15%) species. More than half of the oxidation and all the methylation of As(III) resulted from the manipulation by O2 nanobubbles of microbes responsible for As(III) oxidation and methylation. Hydroxyl radicals were generated during the oxidation of reductive substances at the SWI in darkness, and should be dominant contributors to As(III) abiotic oxidation. X-ray absorption near-edge structure (XANES) spectroscopic analysis demonstrated that surface sediments changed from being sources to acting as sinks of As, due to the formation of Fe-(hydr)oxide. Overall, this study suggests that interfacial O2 nanobubble technology could be a potential method for remediation of sediment As pollution through the manipulation of O2-related microbial and geochemical reactions