Portable microfluidic devices for monitoring antibiotic resistance genes in wastewater

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dc.contributor.authorFeng, Rida
dc.contributor.authorMao, Kang
dc.contributor.authorZhang, Hua
dc.contributor.authorZhu, Hongxiang
dc.contributor.authorDu, Wei
dc.contributor.authorYang, Zhugen
dc.contributor.authorWang, Shuangfei
dc.date.accessioned2025-01-22T12:32:40Z
dc.date.available2025-01-22T12:32:40Z
dc.date.freetoread2025-01-22
dc.date.issued2025-01-31
dc.date.pubOnline2024-12-21
dc.description.abstractAntibiotic resistance genes (ARGs) pose serious threats to environmental and public health, and monitoring ARGs in wastewater is a growing need because wastewater is an important source. Microfluidic devices can integrate basic functional units involved in sample assays on a small chip, through the precise control and manipulation of micro/nanofluids in micro/nanoscale spaces, demonstrating the great potential of ARGs detection in wastewater. Here, we (1) summarize the state of the art in microfluidics for recognizing ARGs, (2) determine the strengths and weaknesses of portable microfluidic chips, and (3) assess the potential of portable microfluidic chips to detect ARGs in wastewater. Isothermal nucleic acid amplification and CRISPR/Cas are two commonly used identification elements for the microfluidic detection of ARGs. The former has better sensitivity due to amplification, but false positives due to inappropriate primer design and contamination; the latter has better specificity. The combination of the two can achieve complementarity to a certain extent. Compared with traditional microfluidic chips, low-cost and biocompatible paper-based microfluidics is a very attractive test for ARGs, whose fluid flow in paper does not require external force, but it is weaker in terms of repeatability and high-throughput detection. Due to that only a handful of portable microfluidics detect ARGs in wastewater, fabricating high-throughput microfluidic chips, developing and optimizing recognition techniques for the highly selective and sensitive identification and quantification of a wide range of ARGs in complex wastewater matrices are needed.
dc.description.journalNameMicrochimica Acta
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (42377456), Guizhou Provincial Science and Technology Projects (Qiankehe Jichu-ZK [2022] Yiban 565, Qiankehe Platform Talents-GCC [2023] 046), the Hebei Provincial Science and Technology Projects (24291703Z), and the Youth Innovation Promotion Association CAS (2023415), CAS-ANSO Fellowship (CAS-ANSO-FS-2024–34).
dc.format.mediumElectronic
dc.identifier.citationFeng R, Mao K, Zhang H, et al., (2025) Portable microfluidic devices for monitoring antibiotic resistance genes in wastewater. Microchimica Acta, Volume 192, Issue 1, January 2025, Article number 19
dc.identifier.eissn1436-5073
dc.identifier.elementsID561698
dc.identifier.issn0026-3672
dc.identifier.issueNo1
dc.identifier.paperNo19
dc.identifier.urihttps://doi.org/10.1007/s00604-024-06898-w
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/23416
dc.identifier.volumeNo192
dc.languageEnglish
dc.language.isoen
dc.publisherSpringer
dc.publisher.urihttps://link.springer.com/article/10.1007/s00604-024-06898-w
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAntibiotic resistance genes (ARGs)
dc.subjectMicrofluidics
dc.subjectIsothermal nucleic acid amplification
dc.subjectCRISPR/Cas
dc.subjectWastewater analysis
dc.subject3401 Analytical Chemistry
dc.subject34 Chemical Sciences
dc.subjectBioengineering
dc.subjectBiotechnology
dc.subjectAntibiotic resistance genes (ARGs)
dc.subjectCRISPR/Cas
dc.subjectIsothermal nucleic acid amplification
dc.subjectMicrofluidics
dc.subjectWastewater analysis
dc.subjectAnalytical Chemistry
dc.subject3401 Analytical chemistry
dc.subject.meshWastewater
dc.subject.meshLab-On-A-Chip Devices
dc.subject.meshNucleic Acid Amplification Techniques
dc.subject.meshDrug Resistance, Microbial
dc.subject.meshGenes, Bacterial
dc.subject.meshCRISPR-Cas Systems
dc.subject.meshAnti-Bacterial Agents
dc.subject.meshMicrofluidic Analytical Techniques
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshAnti-Bacterial Agents
dc.subject.meshMicrofluidic Analytical Techniques
dc.subject.meshNucleic Acid Amplification Techniques
dc.subject.meshDrug Resistance, Microbial
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshGenes, Bacterial
dc.subject.meshLab-On-A-Chip Devices
dc.subject.meshCRISPR-Cas Systems
dc.subject.meshWastewater
dc.subject.meshWastewater
dc.subject.meshLab-On-A-Chip Devices
dc.subject.meshNucleic Acid Amplification Techniques
dc.subject.meshDrug Resistance, Microbial
dc.subject.meshGenes, Bacterial
dc.subject.meshCRISPR-Cas Systems
dc.subject.meshAnti-Bacterial Agents
dc.subject.meshMicrofluidic Analytical Techniques
dc.subject.meshDrug Resistance, Bacterial
dc.titlePortable microfluidic devices for monitoring antibiotic resistance genes in wastewater
dc.typeArticle
dc.type.subtypeReview
dcterms.dateAccepted2024-12-13

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