Amperometric biosensor for formic acid in air
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Sandström, K. J. Mattias
Newman, Jeffrey D.
Sunesson, Anna-Lena
Levina, Jan-Olof
Turner, Anthony P. F.
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0925-4005
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K. J. Mattias Sandström, Jeffrey Newman, Anna-Lena Sunesson, Jan-Olof Levina, and Anthony P. F. Turner, Amperometric biosensor for formic acid in air, Sensors and Actuators B, Volume 70, Issues 1-3, 1 November 2000, Pages 182-187
Abstract
The possibility of developing a simple, inexpensive and specific personal passive ”real-time” air sampler based on biosensor technology was investigated. Formic acid was used as a model substance. The sensor is based on the enzymatic reaction between formic acid and formate dehydrogenase with NAD+ as a cofactor and Meldola’s blue as mediator. An effective way to immobilise the enzyme, cofactor and Meldola’s blue on screen-printed electrodes was found to be in a mixture of glycerol and phosphate buffer covered with a gas-permeable membrane. When the sensor was introduced into an atmosphere containing formic acid, it gave a distinct and rapid amperometric response.