Kinetic study of the biodegradation of acephate by indigenous soil bacterial isolates in the presence of humic acid and metal ions

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dc.contributor.authorSingh, Simranjeet
dc.contributor.authorKumar, Vijay
dc.contributor.authorSingla, Sourav
dc.contributor.authorSharma, Minaxi
dc.contributor.authorSingh, Dhananjaya P.
dc.contributor.authorPrasad, Ram
dc.contributor.authorThakur, Vijay Kumar
dc.contributor.authorSingh, Joginder
dc.date.accessioned2020-07-30T13:52:52Z
dc.date.available2020-07-30T13:52:52Z
dc.date.issued2020-03-11
dc.description.abstractMany bacteria have the potential to use specific pesticides as a source of carbon, phosphorous, nitrogen and sulphur. Acephate degradation by microbes is considered to be a safe and effective method. The overall aim of the present study was to identify acephate biodegrading microorganisms and to investigate the degradation rates of acephate under the stress of humic acid and most common metal ions Fe(III) and copper Cu(II). Pseudomonas azotoformanss strain ACP1, Pseudomonas aeruginosa strain ACP2, and Pseudomonas putida ACP3 were isolated from acephate contaminated soils. Acephate of concentration 100 ppm was incubated with separate strain inoculums and periodic samples were drawn for UV—visible, FTIR (Fourier-transform infrared spectroscopy) and MS (Mass Spectrometry) analysis. Methamidophos, S-methyl O-hydrogen phosphorothioamidate, phosphenothioic S-acid, and phosphenamide were the major metabolites formed during the degradation of acephate. The rate of degradation was applied using pseudo-first-order kinetics to calculate the half-life (t1/2) values, which were 14.33–16.72 d−1 (strain(s) + acephate), 18.81–21.50 d−1 (strain(s) + acephate + Cu(II)), 20.06 –23.15 d−1 (strain(s) + acephate + Fe(II)), and 15.05–17.70 d−1 (strains + acephate + HA). The biodegradation efficiency of the three bacterial strains can be ordered as P. aeruginosa > P. putida > P. azotoformans. The present study illustrated the decomposition mechanism of acephate under different conditions, and the same may be applied to the removal of other xenobiotic compoundsen_UK
dc.identifier.citationSingh S, Kumar V, Singla S, et al., (2020) Kinetic study of the biodegradation of acephate by indigenous soil bacterial isolates in the presence of humic acid and metal ions. Biomolecules, Volume 10, Issue 3, March 2020, Article number 433en_UK
dc.identifier.issn2218-273X
dc.identifier.urihttps://doi.org/10.3390/biom10030433
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/15616
dc.language.isoenen_UK
dc.publisherMDPIen_UK
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjecttoxicityen_UK
dc.subjectPseudomonasen_UK
dc.subjectmetal stressen_UK
dc.subjecthumic aciden_UK
dc.subjectacephateen_UK
dc.titleKinetic study of the biodegradation of acephate by indigenous soil bacterial isolates in the presence of humic acid and metal ionsen_UK
dc.typeArticleen_UK

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