Advanced lignocellulose bioprocessing for Aloe vera leaf rind through novel termite gut microbiome consortia for acetone butanol ethanol (ABE) production: metagenomics insights and process economic analysis

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dc.contributor.authorRajeswari, Gunasekaran
dc.contributor.authorKumar, Vinod
dc.contributor.authorJacob, Samuel
dc.date.accessioned2025-07-16T11:58:11Z
dc.date.available2025-07-16T11:58:11Z
dc.date.freetoread2025-07-16
dc.date.issued2025-08-01
dc.date.pubOnline2025-07-04
dc.descriptionThe 16S rRNA and ITS raw sequence reads of the termite microbiome and ETC-3 were submitted to the NCBI, with the accession numbers provided under the Bioproject PRJNA1182483.
dc.description.abstractConsolidated bioprocessing (CBP) of lignocellulosic biomass (LCB) using microbes simplifies the process, eliminates enzyme cost and reduces the overall processing expenses. In this regard, termite gut, a potent reservoir of microbial symbionts produces various lignocellulolytic enzymes which acts synergistically to degrade LCB. However, the effectiveness of adapting the microbes with LCB for improved lignocellulolytic enzyme secretion and substrate degradation has been overlooked. Hence, in this study adaptive laboratory (ALE) of termite gut isolates was performed with various substrates such as saw dust (SD) and Aloe vera leaf rind (AVLR) under different conditions. Among the consortia, enriched termite consortium (ETC-3) showed the highest degradation of lignin (51.86 ± 2.03 %, w/w), hemicellulose (29.27 ± 1.29 %, w/w) and cellulose (41.97 ± 2.99 %, w/w) with maximum specific enzyme activities. High throughput sequencing revealed the significant enrichment of Proteobacteria (88.95 %) and Ascomycota (99.94 %) groups in ETC-3. Further, the efficiency of ETC-3 in consolidated pretreatment and bioprocessing (CPBP) and CBP of AVLR towards acetone, butanol and ethanol (ABE) production was studied. Compared to the CPBP, CBP resulted in 1.6-fold higher glucose yield which subsequently enhanced the butanol yield (7.97 ± 0.40 g/L). Finally, cost benefit analysis ensured the economic feasibility of process strategies for AVLR valorization.
dc.description.journalNameInternational Journal of Biological Macromolecules
dc.identifier.citationRajeswari G, Kumar V, Jacob S. (2025) Advanced lignocellulose bioprocessing for Aloe vera leaf rind through novel termite gut microbiome consortia for acetone butanol ethanol (ABE) production: metagenomics insights and process economic analysis. International Journal of Biological Macromolecules, Volume 319, Part 4, August 2025, Article number 145691en_UK
dc.identifier.elementsID673948
dc.identifier.issn0141-8130
dc.identifier.paperNo145691
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2025.145691
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/24189
dc.identifier.volumeNo319, Part 4
dc.languageEnglish
dc.language.isoen
dc.publisherElsevieren_UK
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0141813025062464?via%3Dihub
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject31 Biological Sciencesen_UK
dc.subject3106 Industrial Biotechnologyen_UK
dc.subjectPolymersen_UK
dc.subject3101 Biochemistry and cell biologyen_UK
dc.subjectAloe vera leaf rinden_UK
dc.subjectBiorefineryen_UK
dc.subjectLignocellulose degradationen_UK
dc.subjectMicrobial consortiumen_UK
dc.titleAdvanced lignocellulose bioprocessing for Aloe vera leaf rind through novel termite gut microbiome consortia for acetone butanol ethanol (ABE) production: metagenomics insights and process economic analysisen_UK
dc.typeArticle
dcterms.dateAccepted2025-06-30

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