A new reactive polymer suitable for covalent immobilisation and monitoring of primary amines

Abstract

A new polymer able to react with primary amines was synthesised from allyl mercaptan, o-phtalic dialdehyde and ethylene glycol dimethacrylate by radical polymerisation. Reactive thioacetale formed by allyl mercaptan and dialdehyde can bind primary amino groups without additional pre-activation forming fluorescent isoindole complex. It gives a great opportunity to monitor binding and loading of the amino compounds on the reactive surface. The reactive polymer is found to be an effective matrix for immobilisation of the proteins and other amino containing compounds in affinity chromatography and could be used for their detection in solution.