Development of immunosensors for mycotoxins analysis

Abstract

Aflatoxin B₁ (AFB₁) and fumonisins (Fms) are mycotoxin contaminants found in peanuts and corn, respectively, and are known to be immunosuppressive and carcinogenic compounds. Therefore, the development of rapid and sensitive method for detecting these toxins especially for field analysis is required for risk assessment and management. The work presented in this thesis reports on the construction of sensor platforms capable of fulfilling these requirements. The use of, screen-printed thick film electrodes, gold nano-particle application and microelectrodes on a silicone support were investigated as suitable sensor platforms. The development of indirect and direct competitive immunoassay formats for the electrochemical immunosensor construction was undertaken for AFB₁ and Fms determination. Cont/d.