Tunable conjugated polymers for bacterial differentiation

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dc.contributor.authorGolabi, Mohsen
dc.contributor.authorTurner, Anthony P. F.
dc.contributor.authorJager, Edwin W. H.
dc.date.accessioned2020-10-20T11:21:40Z
dc.date.available2020-10-20T11:21:40Z
dc.date.issued2015-09-08
dc.description.abstractA novel rapid method for bacterial differentiation is explored based on the specific adhesion pattern of bacterial strains to tunable polymer surfaces. Different types of counter ions were used to electrochemically fabricate dissimilar polypyrrole (PPy) films with diverse physicochemical properties such as hydrophobicity, thickness and roughness. These were then modulated into three different oxidation states in each case. The dissimilar sets of conducting polymers were exposed to five different bacterial strains, Deinococcus proteolyticus, Serratia marcescens, Pseudomonas fluorescens, Alcaligenes faecalis and Staphylococcus epidermidis. By analysis of the fluorescent microscope images, the number of bacterial cells adhered to each surface were evaluated. Generally, the number of cells of a particular bacterial strain that adhered varied when exposed to dissimilar polymer surfaces, due to the effects of the surface properties of the polymer on bacterial attachment. Similarly, the number of cells that adhered varied with different bacterial strains exposed to the same surface, reflecting the different surface properties of the bacteria. Principal component analysis showed that each strain of bacteria had its own specific adhesion pattern. Hence, they could be discriminated by this simple, label-free method based on tunable polymer arrays combined with pattern recognition.en_UK
dc.identifier.citationGolabi M, Turner A , Jager E. (2016) Tunable conjugated polymers for bacterial differentiation. Sensors and Actuators B: Chemical, Volume 222, January 2016, pp. 839-848en_UK
dc.identifier.issn0925-4005
dc.identifier.urihttps://doi.org/10.1016/j.snb.2015.09.033
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/15905
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPrincipal component analysis (PCA)en_UK
dc.subjectPattern recognitionen_UK
dc.subjectBacterial adhesionen_UK
dc.subjectRapid microbial detectionen_UK
dc.subjectPolypyrroleen_UK
dc.subjectConducting polymeren_UK
dc.titleTunable conjugated polymers for bacterial differentiationen_UK
dc.typeArticleen_UK

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