Diversity and bioactivity of endophytic actinobacteria associated with the roots of artemisia herba-alba asso from Algeria

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dc.contributor.authorDjemouai, Nadjette
dc.contributor.authorMeklat, Atika
dc.contributor.authorYoucef, Khadidja Oulad Hadj
dc.contributor.authorNacer, Asma
dc.contributor.authorSaadi, Sid Ahmed
dc.contributor.authorVerheecke-Vaessen, Carol
dc.date.accessioned2024-10-25T15:12:51Z
dc.date.available2024-10-25T15:12:51Z
dc.date.freetoread2024-10-25
dc.date.issued2024-12-01
dc.date.pubOnline2024-10-11
dc.descriptionThe datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request, while 16S rRNA data generated are available in the Genbank repository.
dc.description.abstractThe isolation of endophytic actinobacteria from the roots of wild populations of Artemisia herba-alba Asso, a medicinal plant collected from the arid lands of Algeria, is reported for the first time. Forty-five actinobacterial isolates were identified by molecular analysis and in vitro evaluated for antimicrobial activity and plant growth-promoting (PGP) abilities (1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, nitrogen fixation, phosphate and potassium solubilization, ammonia, and siderophores production). The phylogenetic relationships based on 16S rRNA gene sequences show that the genus Nocardioides (n = 23) was dominant in the sampled localities. The remaining actinobacterial isolates were identified as Promicromonospora (n = 11), Streptomyces (n = 6), Micromonopora (n = 3), and Saccharothrix (n = 2). Only six (13.33%) strains (five Streptomyces and one Saccharothrix species) were antagonistic in vitro against at least one or more indicator microorganisms. The antimicrobial activity of actinobacterial strains targeted mainly Gram-positive bacteria. The results demonstrate that more than 73% of the isolated strains had ACC deaminase activity, could fix atmospheric nitrogen and were producers of ammonia and siderophores. However, only one (2.22%) strain named Saccharothrix sp. BT79 could solubilize phosphorus and potassium. Overall, many strains exhibited a broad spectrum of PGP abilities. Thus, A. herba-alba provides a source of endophytic actinobacteria that should be explored for their potential biological activities.
dc.description.journalNameCurrent Microbiology
dc.description.sponsorshipMinistry of Higher Education and Scientific Research
dc.format.mediumElectronic
dc.identifier.citationDjemouai N, Meklat A, Youcef KOH, et al., (2024) Diversity and bioactivity of endophytic actinobacteria associated with the roots of artemisia herba-alba asso from Algeria. Current Microbiology, Volume 81, Issue 12, December 2024, Article number 402en_UK
dc.identifier.eissn1432-0991
dc.identifier.elementsID554835
dc.identifier.issn0343-8651
dc.identifier.issueNo12
dc.identifier.paperNo402
dc.identifier.urihttps://doi.org/10.1007/s00284-024-03932-1
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/23127
dc.identifier.volumeNo81
dc.languageEnglish
dc.language.isoen
dc.publisherSpringer en_UK
dc.publisher.urihttps://link.springer.com/article/10.1007/s00284-024-03932-1
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject31 Biological Sciencesen_UK
dc.subjectInfectious Diseasesen_UK
dc.subject2 Zero Hungeren_UK
dc.subjectMicrobiologyen_UK
dc.subject.meshAlgeriaen_UK
dc.subject.meshActinobacteriaen_UK
dc.subject.meshPlant Rootsen_UK
dc.subject.meshPhylogenyen_UK
dc.subject.meshEndophytesen_UK
dc.subject.meshArtemisiaen_UK
dc.subject.meshRNA, Ribosomal, 16Sen_UK
dc.subject.meshBiodiversityen_UK
dc.subject.meshNitrogen Fixationen_UK
dc.subject.meshSiderophoresen_UK
dc.titleDiversity and bioactivity of endophytic actinobacteria associated with the roots of artemisia herba-alba asso from Algeriaen_UK
dc.typeArticle
dc.type.subtypeJournal Article
dcterms.dateAccepted2024-10-02

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