Environmental stress and elicitors enhance taxol production by endophytic strains of Paraconiothyrium variabile and Epicoccum nigrum

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dc.contributor.authorSomjaipeng, Supunnika
dc.contributor.authorMedina-Vayá, Ángel
dc.contributor.authorMagan, Naresh
dc.date.accessioned2016-05-16T07:50:36Z
dc.date.available2016-05-16T07:50:36Z
dc.date.issued2016-05-04
dc.description.abstractThis study examined the effect of different elicitors (seven, different concentrations) and environmental factors (water activity (aw), pH) on taxol production by strains of two endophytic fungi, Paraconiothyrium variabile and Epicoccum nigrum, isolated from temperate yew trees. A defined liquid broth medium was modified with elicitors, solute aw depressors at different pH values. For P. variabile, the best elicitor was salicylic acid at 50 mg/l which gave a taxol yield of 14.7 ± 4.8 μg/l. The study of synergistic effects between elicitor, aw and pH on taxol production showed that the highest yield of taxol (68.9 ± 11.9 μg/l) was produced under modified ionic stress of 0.98 aw (KCl) at pH 5 when supplemented with 20 mg/l of salicylic acid. For E. nigrum, serine was the best elicitor which increased yield significantly (29.6 fold) when KCL was used as the aw depressor (0.98 aw) at pH 5.0 with 30 mg/l of serine. The maximum taxol yield produced by E. nigrum was 57.1 ± 11.8 μg/l. Surface response models were used to build contour maps to determine the conditions for maximum and marginal conditions for taxol yield in relation to the best elicitor and aw, and the best pH for the first time. This will be beneficial for identifying key parameters for improvement of taxol yields by endophytic fungi.en_UK
dc.identifier.citationSupunnika Somjaipeng, Angel Medina, Naresh Magan, Environmental stress and elicitors enhance taxol production by endophytic strains of Paraconiothyrium variabile and Epicoccum nigrum, Enzyme and Microbial Technology, Volume 90, August 2016, pp69-75en_UK
dc.identifier.issn0141-0229
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/9895
dc.identifier.urihttp://dx.doi.org/10.1016/j.enzmictec.2016.05.002
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectElicitorsen_UK
dc.subjectWater stressen_UK
dc.subjectpHen_UK
dc.subjectGrowthen_UK
dc.subjectTaxol productionen_UK
dc.subjectEnhanced productionen_UK
dc.subjectPharma productsen_UK
dc.titleEnvironmental stress and elicitors enhance taxol production by endophytic strains of Paraconiothyrium variabile and Epicoccum nigrumen_UK
dc.typeArticleen_UK

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