Effects of parental exposure to amitriptyline on the survival, development, behavior, and gene expression in zebrafish offspring

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dc.contributor.authorLiu, Anqi
dc.contributor.authorChen, Chen
dc.contributor.authorChen, Kun
dc.contributor.authorShi, Yanhong
dc.contributor.authorGrabowski, Robert C.
dc.contributor.authorQiu, Xuchun
dc.date.accessioned2023-12-13T14:27:11Z
dc.date.available2023-12-13T14:27:11Z
dc.date.freetoread2024-12-07
dc.date.issued2023-12-06
dc.description.abstractIn mammals, parental exposure to amitriptyline (AMI) has been proven to contribute to congenital disabilities in their offspring. However, no studies have paid attention to the adverse effects of parental exposure to amitriptyline on fish offspring. In this study, we exposed adult zebrafish (F0) to AMI (0.8 μg/L) for 21 days. Subsequently, these zebrafish (F0) were allowed to mate, and their offspring (F1) were collected to culture in clean water for 5 days. The mortality rate, average hatching time, and heart rate at 48 h post-fertilization (hpf) of F1 were investigated. Our results showed that parental exposure to AMI induced tachycardia and increased mortality in F1 zebrafish. Under a light/dark transition test, F1 larvae born from AMI-exposed parents exhibited lower locomotor activity in the dark period and decreased thigmotaxis in the light period. The transcriptome analysis showed that parental AMI exposure dysregulated some key pathways in their offspring. Through the prediction of key driver analysis, six differentially expressed genes (DEGs) were revealed as key driver genes involved in protein processing in endoplasmic reticulum (hspa5, hsp70.1, hsp90a), ribosome (rps27a) and PPAR signaling pathway (pparab and fabp2). Considering that the concentration of AMI residual components in natural water bodies may be over our test concentration (0.8 μg/L), our findings suggested that toxicity of parental exposure to the offspring of fish should receive greater attention.en_UK
dc.identifier.citationLiu A, Chen C, Chen K, et al., (2024) Effects of parental exposure to amitriptyline on the survival, development, behavior, and gene expression in zebrafish offspring, Science of the Total Environment, Volume 912, February 2024 Article number 169173en_UK
dc.identifier.eissn1879-1026
dc.identifier.issn0048-9697
dc.identifier.urihttps://doi.org/10.1016/j.scitotenv.2023.169173
dc.identifier.urihttps://dspace.lib.cranfield.ac.uk/handle/1826/20590
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectantidepressanten_UK
dc.subjectbehavior responsesen_UK
dc.subjectkey driver analysisen_UK
dc.subjectparental exposureen_UK
dc.subjectzebrafishen_UK
dc.titleEffects of parental exposure to amitriptyline on the survival, development, behavior, and gene expression in zebrafish offspringen_UK
dc.typeArticleen_UK
dcterms.dateAccepted2023-12-05

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